Early access now open

HEK293 Secreted Expression Check.

Fast mammalian expression data for designed secreted proteins, before you spend time and budget on purification or custom assays.

Request Early Access View Pricing

Test whether your designs express before committing to custom assay work.

Send a set of designed soluble proteins and receive a standardized mammalian expression readout from HEK293/Expi293 cells.

Use it to rank variants, remove obvious failures, and decide what deserves deeper characterization.

  • Best fit: antibody fragments, miniproteins, binders, enzymes, cytokine-like constructs, and soluble design libraries.
  • Output: secretion/expression titer or relative titer, basic QC flag, raw data, and run metadata.
  • Terms: RUO only. You own your submitted sequences and output data.

What You Get

A standardized screen for teams that need a clean first mammalian expression readout.

Included

  • HEK293 / Expi293 transient expression
  • Secreted soluble proteins only
  • Standard provider vector, promoter, signal peptide, and C-terminal His or Fc tag
  • Standard small-scale run and harvest day
  • Standard controls on every run
  • Secretion/expression titer or relative titer
  • Raw data, run metadata, and basic QC flag

Out of Scope

  • Membrane proteins, custom cell lines, or custom harvest days
  • Custom functional assays or custom purification methods
  • Guaranteed purified yield
  • Clinical, GMP, diagnostic, regulatory, or therapeutic-use work
  • Custom legal terms for early-access runs

Result Package

Each run is delivered as a compact report plus machine-readable data so your team can rank variants, understand failures, and choose next experiments quickly.

01

Variant Ranking

Per-variant expression readout, relative rank, replicate summary, and pass / low / failed-expression flag.

02

QC & Run Notes

Controls, viability/transfection context, plate-map traceability, deviations, and likely failure-mode notes.

03

Data & Interpretation

CSV export with raw and normalized values, run metadata, well-to-variant traceability, and a short scientist note.

Early-Access Pricing

Choose the run size that matches the number of variants you want to screen in the next 90 days.

Starter Run

$495 / variant

For teams ready to screen 24 variants under standard conditions.

Request access
Shared Run

$375 / variant

Volume pricing for 96+ variants in a shared expression run.

Reserve interest
Premium QC

$950 / variant

Expression plus one-step capture and conditional SEC/aggregation QC.

Discuss fit

From Sequence Set To Ranked Variants

PhysioForge turns variant sets into comparable mammalian expression data using a standard intake, run, and reporting format.

01

Submit Variants

Share the protein class, sequence set size, tag preference, and timing needs.

02

Run HEK293 Screen

Variants are expressed under standard HEK293/Expi293 conditions.

03

Receive Results

Get expression readouts, QC flags, raw data, and run metadata for prioritization.

Early access

Request a run.

Tell us what you want to screen. We will reply with fit, timing, and next steps.

RUO posture: Customer sequences and output data remain customer-owned. PhysioForge will not train models on customer sequences without opt-in.

This opens a prefilled email to max@physioforge.bio so we can reply directly.

Accelerating the Next Generation of Therapeutics

PhysioForge is building mammalian validation infrastructure for computational protein design teams.

Early access is best for teams with 24+ secreted soluble protein variants that can use standard HEK293/Expi293 conditions.

Operations: Greater Washington D.C. Biotech Hub